Simulation

Simulate diffusing and photobleaching PSFs with spotSimulator or generate a model image of simple cell shaped objects with 3Dshapes.

3D shapes

Integrate a model 3D PSF over 3D spheres, cylinders etc. to generate model cell like images.

Point Spread function

Generate a measured or simulated PSF. This code will generate a good PSF. Make sure each Z-plane is normalised to unitary intensity and the PSF array is the same size or larger than the image in all 3D.