Single molecule tools

A series of Matlab functions and scripts for tracking and quantification of spots in images, Copy Number deconvolution, segmentation and simulation.

Useful for analysing localisation microscopy, step-wise photobleaching , STORM, PALM, SHRIMP, FRAP and regular fluorescence microscopy data.

See getting started, main function pages for individual tasks (tracking,characteristic intensity,stoichiometry, diffusion, colocalisation deconvolution, segmentation and simulation) or head here for full instructions to analyse step-wise photobleach data.